This document describes the HybriZAP-2.1 Two-Hybrid Predigested Vector/Gigapack Cloning Kit, which includes a predigested vector, Gigapack III Gold packaging extract, and XL1-Blue subcloning-grade competent cells. The product is for research use only and not for use in diagnostic procedures. The document also provides ordering information and technical service contact details.
This document is an instruction manual for the Agilent HybriZAP-2.1 Two-Hybrid Predigested Vector/Gigapack Cloning Kit (Catalog #235611). The kit includes predigested vectors with Gigapack III Gold Packaging Extract and XL1-Blue Subcloning-Grade Competent Cells for creating two-hybrid libraries.
| Vector Predigestion Sites | EcoR I and Xho I |
|---|---|
| Competent Cells Type | XL1-Blue subcloning-grade |
| Packaging Extract Type | Gigapack III Gold |
The XLOLR strain should be used for plating excised phagemids.
The XL1-Blue MRF' strain should be used for all other manipulations.