AffinityScript Multiple Temperature Reverse Transcriptase is a genetically engineered version of MMLV reverse transcriptase that exhibits improved specific activity over a broad range of cDNA synthesis temperatures from 42°C to 55° C. In addition to being more versatile compared to other reverse transcriptases, AffinityScript reverse transcriptase produces higher cDNA yields and provides superior performance in RT-PCR.
This document is a product catalog entry for the Agilent AffinityScript Multiple Temperature Reverse Transcriptase (Catalog Number 600105). It describes the product as a genetically engineered MMLV reverse transcriptase optimized for specific activity between 42°C and 55°C, suitable for synthesizing cDNA from RNA templates ranging from 0.5 to 9.0 kb in size.
The product must be shipped on dry ice and stored at -20°C upon receipt.
The enzyme performs optimally over a broad range of cDNA synthesis temperatures from 42°C to 55°C.
The final reaction volume must be exactly 20 ul.