This instruction manual introduces the use of NOSdetect Assay Kit products
The Agilent NOSdetect Assay Kit is a simple, sensitive, and specific assay designed to measure nitric oxide synthase (NOS) activity. It relies on the biochemical conversion of L-arginine to L-citrulline by NOS, utilizing radioactive substrates like [3H]arginine or [14C]arginine for high sensitivity at the picomole level.
The manual details procedures for preparing extracts from various tissues and cultured cells, separating subcellular fractions, and performing the measurement of NOS activity in enzyme extracts. It includes specific instructions on incubation times based on tissue type and references to additional materials required for the assay.
| Product Name | NOSdetect Assay Kit |
|---|---|
| Catalog Number | 204500 |
| Calmodulin Concentration | 1 uM |
| Reaction Buffer Concentration | 2x |
| N,,-nitro-L-arginine methyl ester HCI Concentration | 10 mM |
| Homogenization Buffer Concentration | 10x |
It is a simple, sensitive and specific assay for nitric oxide synthase (NOS) activity.
The kit is a simple, sensitive and specific assay for nitric oxide synthase (NOS) activity.
Incubations may be carried out for 10-60 minutes depending on the tissue being used.
The reaction should be performed at a temperature between 22°C and 37°C.