This manual describes the use of miRNA QPCR Master Mix
The document provides instructions for using the Agilent miRNA QPCR Master Mix to amplify cDNA templates derived from microRNAs. The kit includes a master mix containing EvaGreen dye and a reference dye, along with a universal reverse primer provided in a separate synthesis kit.
Key sections include materials provided, storage conditions at -20°C or 4°C after thawing, additional required materials like the miRNA 1st-Strand cDNA Synthesis Kit, and detailed preprotocol considerations such as forward primer selection and recommended controls (No Template Control and No-PAP Control). The manual also details data acquisition settings for various spectrofluorometric thermal cyclers.
| Quantity per kit | Sufficient for two hundred 25-uL QPCR reactions |
|---|---|
| Master Mix Concentration | 2x |
| Reference Dye Concentration | 1 mM |
| Master Mix Volume | 2.5 ml |
| Reference Dye Volume | 100 ul |
| EvaGreen Excitation Maximum | 500 nm |
| EvaGreen Emission Maximum | 530 nm |
| Reference Dye Excitation Wavelength | 584 nm |
| Reference Dye Emission Wavelength | 612 nm |
Store at -20°C upon receipt. After thawing, it may be stored at 4°C for up to six months.
Use the reference dye at a final concentration of 30 nM.
Use the reference dye at a final concentration of 300 nM.
No, it is licensed for research and development only.