Agilent Technologies ZAP Express Predigested Vector Kit Instruction Manual

This document describes the features and characteristics of ZAP Express Predigested Vector Kit and ZAP Express Predigested Gigapack Cloning Kit.

Brand: Agilent
Document type: User Manual
Language: English
Pages: 36
File format: PDF
File size: 373 KB
Published: 06 June, 2012
Updated: 28 September, 2023
MD5 checksum: 12D3E5040067D0249C73B00E6E7084B9
PDF: Agilent Technologies ZAP Express Predigested Vector Kit Instruction Manual PDF

About this manual

This document serves as an instruction manual for the Agilent Technologies ZAP Express Predigested Vector Kit (Catalog #239212) and the ZAP Express Predigested Gigapack Cloning Kits (Catalog #239615). These kits are designed for BamH I/CIAP-treated DNA inserts and include essential materials such as digested vectors, bacterial host strains (XL1-Blue MRF' and XLOLR), helper phages (ExAssist and R408), and packaging extracts.

What's inside

Materials Provided
Storage Conditions
Bacterial Host Strains
Ligating the Insert
Packaging and Titering
Plating for Blue-White Color Selection
Amplifying the ZAP Express Library
Hybridizing and Screening
In Vivo Excision of the pBK-CMV Phagemid Vector
Troubleshooting

Specifications

Vector Digestion Treatment	BamH I, CIAP treated
Helper Phage Titer (ExAssist)	~1.0 x 10^7 pfu/ml
Vector Storage Temperature	-20°C

Frequently asked questions

Which bacterial strain is recommended for plating excised phagemids?

The XLOLR strain is recommended for plating excised phagemids.

Which bacterial strain should be used for all other manipulations?

The XL1-Blue MRF' strain is recommended for all other manipulations.

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