The miRNA 1st-Strand cDNA Synthesis Kit is a kit used for converting miRNA into cDNA. The kit has unique features and characteristics and is intended for research use only, not for diagnostic procedures.
The Agilent miRNA 1st-Strand cDNA Synthesis Kit provides reagents to elongate miRNAs in a polyadenylation reaction using E. coli Poly A Polymerase (PAP) and then reverse transcribe the polyadenylated RNA into QPCR-ready cDNA. The kit includes specific enzymes, buffers, primers, and dNTPs necessary for these steps.
| Input RNA Range | 30 ng to 1 ug |
|---|---|
| Reactions Supported | Fifty 20-ul reactions |
| Storage Temperature | -20°C |
RNA samples with OD260/280 ratios of 1.8-2.0 are optimally pure.
No, enriching the RNA sample for miRNA is not necessary but may improve detection of some difficult targets.
If MnCl2 is included in the polyadenylation reaction, purify the polyadenylated RNA with a phenol-chloroform extraction and ethanol precipitation before cDNA synthesis.