This manual provides instructions for using the LumiMax Superoxide Anion Detection Kit. The kit is used to detect the concentration of superoxide anions in cell culture supernatants.
The Agilent LumiMax Superoxide Anion Detection Kit is designed to qualitatively detect the presence of superoxide anions and superoxide dismutase (SOD) in cell cultures with higher sensitivity than standard luminol-based assays. The kit includes specific reagents such as enhancer solution, luminol solution, xanthine solution, and various assay media optimized for tissue culture cells.
| Enhancer solution concentration | 5.0 mM |
|---|---|
| Luminol solution concentration | 4.0 mM |
| Xanthine solution concentration | 1.0 mM |
| Superoxide dismutase (SOD) concentration | 25 U/ul |
| Xanthine oxidase concentration | 0.02 U/ul |
Superoxide dismutase must be stored at -20°C.
All other components should be stored at 4°C.
No, PMA is a potent carcinogen and requires following the manufacturer's safety recommendations.