This manual provides instructions for using Mitochondrial Membrane Potential Detection Kit, including product composition, usage methods, storage conditions, etc.
The Mitochondrial Membrane Potential Detection Kit uses a unique fluorescent cationic dye, JC-1, to signal the loss of mitochondrial membrane potential associated with cellular apoptosis. In healthy non-apoptotic cells, the dye stains mitochondria bright red (J-aggregates), whereas in apoptotic cells where the potential collapses, the dye remains in the cytoplasm as a green fluorescent monomeric form.
| Reactions per kit | 100 reactions |
|---|---|
| JC-1 storage temperature | -20°C |
| Reconstituted JC-1 activity duration | 6 months |
| JC-1 stock solution concentration | 100x |
The kit supports flow cytometry, fluorescence microscopy, and a fluorescent 96-well plate reader format.
Store the lyophilized reagent at -20°C protected from light and moisture. Once reconstituted, store aliquots away from moisture and light.
Cells should be cultured to a density of 1 x 10^6 cells/ml or less.