Agilent Mammalian Two-Hybrid Assay Kit Instruction Manual

This is an instruction manual for Agilent's Mammalian Two-Hybrid Assay Kit


Agilent Mammalian Two-Hybrid Assay Kit Instruction Manual - cover page
Brand
Agilent
Document type
User Manual
Language
English
Pages
27
File format
PDF
File size
199 KB
Published
06 June, 2012
Updated
30 September, 2023
MD5 checksum
93ABE5A6024E6A838D6B8320C7C82F1B

About this manual

The document serves as an instruction manual for the Agilent Mammalian Two-Hybrid Assay Kit, which includes pCMV-BD and pCMV-AD vectors along with control plasmids. It outlines the materials provided, storage conditions, and additional reagents required to perform protein interaction assays.

What's inside

  • Materials Provided
  • Storage Conditions
  • Additional Materials Required
  • Choosing a Cell Line
  • Cloning Considerations
  • Preparing Plasmids
  • Cell Culture and Transfection
  • Extracting Luciferase
  • Performing the Assay
  • Troubleshooting

Specifications

Catalog Number#211344
Vector Concentration1 µg/µl in TE buffer
Storage Temperature-20°C

Frequently asked questions

What cell lines are suitable for this assay?

Suitable mammalian cells include HeLa, CHO, CV-1, COS, 293 and NIH3T3.

What is the storage condition for the kit components?

All components should be stored at -20°C.

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