This document is the instruction manual for Agilent's pMyr XR Vector Kit, providing information on usage limitations, warranty terms, ordering information, and technical service contact details.
The document serves as an instruction manual for the Agilent pMyr XR Vector Kit, a component of the CytoTrap two-hybrid system used for detecting protein-protein interactions in vivo. The kit includes the pMyr XR vector, digested with EcoR I-Xho I and treated with calf intestinal alkaline phosphatase (CIAP), along with an XR LacZ test insert and the cdc25H host strain.
The manual details the preparation of the yeast host strain, including establishing agar plate streaks and preparing -80°C glycerol stocks. It also covers target library construction, vector construction, ligating cDNA into the pMyr XR vector, and determining transformant numbers through color screening on prepared media.
| Vector Insert Size | 600 bp |
|---|---|
| Vector Origin of Replication | pUC and 2 origins |
Vectors should be stored at -20°C.
Yeast strains should be stored at -80°C.