AffinityScript Multiple Temperature Reverse Transcriptase is a genetically engineered version of MMLV reverse transcriptase that exhibits improved specific activity over a broad range of cDNA synthesis temperatures from 42°C to 55° C. In addition to being more versatile compared to other reverse transcriptases, AffinityScript reverse transcriptase produces higher cDNA yields and provides superior performance in RT-PCR.
This document provides a certificate of analysis and usage instructions for the AffinityScript Multiple Temperature Reverse Transcriptase, catalog number 600107. It is a genetically engineered version of MMLV reverse transcriptase designed to produce higher cDNA yields with improved specific activity over temperatures ranging from 42°C to 55°C.
| Temperature Range | 42°C to 55°C |
|---|---|
| Reaction Volume | 20 ul |
| Incubation Time | 1 hour |
| Inactivation Temperature | 70°C |
Store the product at -20°C upon receipt.
Inactivate the reaction by incubating it at 70°C for 15 minutes.