Agilent CytoTrap XR Library Construction Kit Instruction Manual

This document is the CytoTrap XR Library Construction Kit instruction manual, which introduces the product's use methods, maintenance methods, etc.


Agilent CytoTrap XR Library Construction Kit Instruction Manual - cover page
Brand
Agilent
Document type
User Manual
Language
English
Pages
75
File format
PDF
File size
743 KB
Published
06 June, 2012
Updated
30 September, 2023
MD5 checksum
4D67BC60B0CEA22502E4182B6212E838

About this manual

The document serves as an instruction manual for the Agilent CytoTrap XR Library Construction Kit (Catalog #200444). It details the materials provided within the kit, storage conditions for reagents and host strains, and necessary additional equipment. The guide outlines protocols for cDNA synthesis, vector construction, and library preparation.

What's inside

  • Materials Provided
  • Storage Conditions
  • Preparation of Yeast Host Strain
  • cDNA Insert Preparation and Ligation
  • Transforming XL10-Gold Cells
  • Bait Plasmid Construction
  • Yeast Transformation
  • Cotransformation of Bait and Target Plasmids
  • Library Screening
  • Troubleshooting

Specifications

Catalog Number200444
RevisionB
Vector Type 1pSos vector, supercoiled

Frequently asked questions

What is the storage temperature for XL10-Gold Kan Ultracompetent Cells?

XL10-Gold Kan Ultracompetent Cells should be stored at -80°C.

How many vector-ligated constructs can the reagents generate?

Enough reagents are included to generate five vector-ligated constructs.

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