Absolutely RNA Nanoprep Kit INSTRUCTION MANUAL is a reagent kit for RNA extraction. The brand of this kit is Agilent and the model is 400753.
The Absolutely RNA Nanoprep Kit allows for the rapid purification of high-quality total RNA from extremely small samples, specifically 1-10^4 cells, or cells harvested by laser capture microdissection (LCM). The unique design utilizes RNA-binding nano-spin cups to recover sample in a very small volume (10 µl), enabling downstream applications without further concentration steps that might degrade the RNA.
The protocol involves lysing cells with a guanidine thiocyanate-containing buffer, mixing with sulfolane, capturing RNA onto a silica-based fiber matrix within a nano-spin cup, and performing optional DNase digestion followed by washes. Pure RNA is finally eluted in a low-ionic-strength buffer.
| Sample Size Range | 1-10^4 cells |
|---|---|
| Recovery Volume | 10 µl |
| Centrifugation Speed | =12,000 x g |
The kit is suitable for up to 1-10^4 cells per sample.
It must be stored at -20°C after reconstitution.