This document is the instruction manual for Agilent Absolutely RNA 96 Microprep Kit, describing the features and characteristics of the product.
The Absolutely RNA 96 Microprep Kit allows for the rapid purification of high-quality total RNA from small samples of cultured cells. The method eliminates toxic phenol-chloroform extractions and time-consuming ethanol precipitations by using a lysis buffer containing guanidine thiocyanate to lyse cells and prevent RNA degradation.
Following cell lysis, the sample is transferred to a 96-well binding plate with a silica-based fiber matrix where RNA binds. Treatment with low-salt wash buffers followed by a DNase digestion step removes contaminating DNA and proteins. Highly pure RNA is finally eluted from the fiber matrix into a collection plate, suitable for downstream applications like cDNA synthesis and PCR.
Once reconstituted, the RNase-free DNase I must be stored at -20°C.
The final DEPC concentration should be 0.1% (v/v) in deionized water.