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- Manuals
- 212
- Brands
- 30+
- Languages
- ZH
- Last added
- 2012-03-28
Featured Drugs manuals
GeneCopoeia EndoFectin™-CHO Transfection Reagent User Manual
This manual describes the patented cationic polymer EndoFectin™‑CHO, designed for high‑efficiency transfection of CHO cells even in the presence of serum. It outlines the reagent’s advantages, such as superior transfection efficiency, high protein expression, serum‑compatible media, low cytotoxicity, and ease of use.
Detailed procedures cover cell preparation, DNA‑reagent complex formation, transient transfection steps for various culture vessels, and stable transfection workflow including selection of resistant clones. Storage conditions, quality control data, and practical tips for optimizing transfection are also provided. This manual is written in Chinese.
Covers: Product Overview · Composition and Storage · Quality Control · Precautions Before Experiment · Transient Transfection Procedure · Stable Transfection Procedure
chicken heat shock protein 70 (HSP-70) elisa immunoassay kit Manual
This manual describes the intended use of the chicken heat‑shock protein 70 (HSP‑70) ELISA kit, which is for quantitative measurement of HSP‑70 in serum, plasma, cell‑culture supernatant or other related biological fluids. The assay principle uses a solid‑phase antibody‑coated microplate, biotinylated detection antibody, HRP‑streptavidin and TMB substrate to generate a colorimetric signal proportional to HSP‑70 concentration.
The kit includes a 96‑well coated plate, lyophilized standards, sample dilution buffer, detection reagents A and B, substrate solution, stop solution and washing buffer. Detailed procedures cover sample collection, reagent preparation, plate loading, incubation times, washing steps, substrate development, stopping the reaction, and reading absorbance at 450 nm. Storage conditions for reagents, shelf‑life, and safety precautions are also provided. This manual is written in Chinese.
Covers: Intended Use · Principle of the Assay · Reagents and Materials · Sample Collection and Storage · Assay Procedure · Washing Procedure
M-MLV Reverse Transcriptase Product Leaflet
M-MLV Reverse Transcriptase (M‑MLV RT) is a recombinant DNA polymerase derived from *E. coli* that synthesizes the first cDNA strand from RNA templates in the presence of primers. The enzyme has been gene‑optimized to remove RNase H activity, providing strong continuous synthesis capability and high sensitivity, allowing up to 8 kb cDNA products from template concentrations of 1 ng–5 µg.
The leaflet lists the enzyme (200 U/µl) together with a 5× reaction buffer, storage solution composition, quality‑control criteria, and a step‑by‑step example protocol for first‑strand cDNA synthesis. It also includes storage recommendations (‑20 °C for up to one year), precautions against RNase contamination, and ordering information for two activity levels. This manual is written in Chinese.
Covers: Product Features · Product Composition · Activity Unit Definition · Enzyme Storage Solution · 5× Reaction Buffer · Quality Control
Maxim MAB-0352 Mouse Anti-Human Granzyme B Monoclonal Antibody User Manual
This manual describes the Maxim MAB-0352 mouse anti‑human Granzyme B monoclonal antibody, a ready‑to‑use (no dilution required) reagent for immunohistochemical staining of formalin‑fixed paraffin‑embedded or frozen tissue sections. The antibody targets human Granzyme B and is intended for research use only, particularly for detecting cytotoxic T‑cells and NK cells.
The document provides product specifications, storage and handling guidelines, incubation conditions, and references for further reading. It also notes that the antibody must be used together with Maxim's immunohistochemistry detection kit to achieve optimal staining results. This manual is written in Chinese.
Covers: Product Overview · Product Specification · Immunoglobulin Type · Physical Characteristics · Product Features · Applications
Latest Drugs manuals
Envirologix transgenosis ELISA kit operating instructions
This document provides information on the testing procedure of the EnviroLogix GMO Plate Kit, including sample preparation, addition of reagents, mixing and incubation, and result interpretation.
melamine test kit Manual
The melamine detection kit is suitable for the quantitative analysis of melamine in contaminated samples.
RNase Inhibitor (RNA Enzyme Inhibitors ) Manual
RNase Inhibitor is an enzyme that can effectively inhibit the activity of RNase A, RNase B and RNase C in eukaryotes. It is widely used in the experiments of cDNA synthesis, in vitro transcription, in vitro expression, mRNA separation and purification with potential RNase contamination.
GeneCopoeia EndoFectin™-CHO Transfection Reagent User Manual
User Manual 2 pages · 495 KB · ZHThe GeneCopoeia EndoFectin™-CHO reagent manual provides product overview, storage guidelines, and step‑by‑step transient and stable transfection protocols.
rat α1 Micro- Ball protein bikunin Precursor (AMBP)ELISA kit User Manual
The rat α1 microglobulin bikunin precursor (AMBP) ELISA kit is used for in vitro quantitative measurement of AMBP content in rat serum, plasma, tissue homogenate, cell culture supernatant or other related liquids.
rat Micro- Tube Related protein 2(MAP-2)ELISA kit Manual
MAP-2 ELISA kit instruction manual, which can be used to quantitatively measure the content of MAP-2 in rat serum, plasma, tissue homogenate, cell culture supernatant or other related liquids.
chicken heat shock protein 70 (HSP-70) elisa immunoassay kit Manual
User Manual 3 pages · 125 KB · ZHThe USCNLIFE E0873Ch ELISA kit manual provides step‑by‑step instructions for measuring chicken HSP‑70 in serum, plasma, or cell‑culture supernatants.
M-MLV Reverse Transcriptase Product Leaflet
Product Leaflet 1 pages · 129 KB · ZHThe M-MLV Reverse Transcriptase product leaflet describes its composition, activity, storage, usage protocol, and ordering details.
Maxim MAB-0352 Mouse Anti-Human Granzyme B Monoclonal Antibody User Manual
User Manual 1 pages · 134 KB · ZHInstructions and specifications for Maxim MAB-0352 mouse anti‑human Granzyme B monoclonal antibody, a ready‑to‑use immunohistochemistry reagent.
eiaab E0640r ELISA Kit User Manual
User Manual 4 pages · 129 KB · ZHThe eiaab E0640r ELISA kit manual provides step‑by‑step instructions for quantifying rat calmodulin (CAM) in serum or plasma.
Rat Hepcidin ELISA Kit User Manual
User Manual 2 pages · 166 KB · ZHInstructions for using the rat Hepcidin ELISA kit to measure Hepcidin levels in serum and plasma samples.
GENMED QuikFusin Cell Transfection Kit User Manual
User Manual 3 pages · 127 KB · ZHThe GENMED QuikFusin Cell Transfection Kit User Manual provides step‑by‑step instructions, reagent details, and safety notes for transfecting various cell…
Beijing Sanjiu Biyanning Keli Product Leaflet
Product Leaflet 2 pages · 41 KB · ZHInstruction leaflet for Biyanning Keli, a Chinese herbal granule for treating rhinitis.
Bogu Biotechnology SGC7901/DDP Cell Line Usage Manual
Product Leaflet 1 pages · 128 KB · ZHThis manual provides composition, required reagents, culture conditions, methods, and troubleshooting for the SGC7901/DDP gastric cancer cisplatin‑resistant…
human Slit2 elisa immune elisa kit Manual
User Manual 5 pages · 428 KB · ZHThe Uscnk E0672 ELISA kit manual provides step‑by‑step instructions for measuring human Slit2 in serum, plasma or tissue samples.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Application Note
How to use the product for one specific task, with worked examples.
Questions answered in Drugs manuals
How should EndoFectin™‑CHO be stored?
Store the sealed reagent at 4 °C – 8 °C; it remains stable for at least 12 months under these conditions.
From: GeneCopoeia EndoFectin™-CHO Transfection Reagent User Manual
What DNA‑to‑reagent ratio is recommended?
Use 3 µl of EndoFectin™ per 1 µg of DNA (range 1‑4 µl per µg can be optimized).
From: GeneCopoeia EndoFectin™-CHO Transfection Reagent User Manual
What types of samples can be tested with this kit?
The kit can be used with chicken serum, plasma, cell‑culture supernatant, or other related biological fluids.
From: chicken heat shock protein 70 (HSP-70) elisa immunoassay kit Manual
What is the detection range of the assay?
The assay detects HSP‑70 concentrations from 0.156 ng/ml up to 10 ng/ml.
From: chicken heat shock protein 70 (HSP-70) elisa immunoassay kit Manual
How is one unit of M‑MLV Reverse Transcriptase defined?
One unit (U) is defined as the amount of enzyme that synthesizes 1 nmol of dTTP into nucleic acid in 10 minutes at 37 °C.
What are the recommended storage conditions for the enzyme?
The enzyme should be stored at –20 °C and remains stable for up to one year.