Large-Volume Injection for Gas Chromatography Using COC-SVE Application Note

About this manual

The document explains that Large-Volume Injection (LVI) is a valuable tool for trace analysis by gas chromatography, enabling injection volumes up to 1 mL. It focuses on the Cool On-Column (COC) inlet combined with a Solvent Vapor Exit (SVE) kit to selectively evaporate bulk solvent before sample transfer.

The text details the hardware configuration involving a precolumn assembly and vent line, as well as the software integration within Agilent ChemStation for automated method development. It provides experimental results demonstrating the technique's effectiveness for analyzing straight-chain hydrocarbons, polycyclic aromatic hydrocarbons (PAHs), and semivolatiles in drinking water extracts.

What's inside

• Introduction
• COC-SVE Hardware Configuration
• Software Integration
• Advantages and Disadvantages
• Experimental Results: PAH Analysis
• Semivolatile Analysis

Frequently asked questions

What is the maximum injection volume achievable with standard pulsed splitless techniques on Agilent 6890 and 5890 Series GCs?

These systems allow approximately two times the normal injection volume, up to 5 uL depending on the solvent.

What is the recommended delay time for the SVE valve when analyzing a diluted drinking water extract with a 25-uL injection?

The recommended delay time is 0.45 minute.

What software version of ChemStation supports LVI with COC-SVE on the G1513A injector?

ChemStation software version 4.04.02 or higher is required.