Empirical Formula Determinations and Compound-Independent Calibration Using a GC-AED System
The document details a study verifying the compound-independent response of the second-generation Agilent G2350A GC-AED system. The system, consisting of an Agilent 6890 Series GC and AED ChemStation software, was tested using mixtures of organochlorine pesticides and other compounds to evaluate its ability to calculate elemental mole ratios (EMR) without specific analyte standards.
The study compared performance at different injection volumes (0.1 µL vs 1.0 µL) and split ratios. Results indicated that while the system generally produces accurate empirical formulas, response factor reproducibility is significantly better with 1.0 µL injections compared to 0.1 µL injections. The note also provides operating conditions for various elements and compares measured minimum detection limits against published specifications.
The data indicates that response factor reproducibility is better for 1.0 µL injections compared to 0.1 µL injections.
The largest error was seen when calibrating on endosulfan sulfate due to a low area on the oxygen chromatogram.