This document describes the application of Agilent 7683 automatic liquid sampler (ALS). ALS can be used to perform thermostatted headspace analysis and ambient headspace analysis. The benefits of using ALS include increased sample throughput, reduced equipment cost, and simplified operation.
Headspace analysis is an alternative to direct injection for samples containing components that might foul the inlet or detector. Ambient headspace sampling with the Agilent 7683 Automatic Liquid Sampler (ALS) offers increased sample throughput, lower equipment costs, and simplified operation compared to temperature-controlled methods.
The document details a typical procedure involving aliquoting liquid or solid samples into vials, agitating them to equilibrate analytes between the sample and air space, and injecting the headspace. It presents three specific examples: determining alcohols in water/blood, analyzing residual solvents in pharmaceuticals per USP 467, and detecting trichloroanisole (TCA) in wine and cork.
| Sample depth | 20 mm |
|---|
It prevents contamination of injectors and columns by nonvolatiles, reduces solvent amount to avoid obscuring early peaks, increases sensitivity for volatile analytes, and allows analysis of volatiles in solid samples.
Do not fill the vial more than half full.
The recommended volume is 0.1 to 1 mL.
The typical sample depth is 20 mm.