Agilent Manuals

The document describes a fast method for the analysis of synthetic antioxidants in vegetable oils. The method uses the Agilent 1290 Infinity LC system equipped with an Agilent ZORBAX Rapid Resolution High Definition (RRHD) column and achieves high throughput in 2 minutes with a backpressure of 1120 bar, which is below the 1200 bar upper limit of the column. Optimization of the mobile phase composition and the temperature are discussed. The figures of merit are illustrated using standard solutions and spiked vegetable oil (sunflower, rapeseed, and olive) extracts. Limits of detection are 1 mg/kg or less in the oil samples. Using a simple methanol extraction, good recovery was obtained for all antioxidants in the oil samples.

This application note describes a method for high-resolution analysis of intact triglycerides under non-aqueous reversed phase gradient conditions using the Agilent 1290 Infinity LC System.

Using Agilent Pesticides Application Kit can quickly and efficiently establish multi-residue pesticide LC/MS/MS methods

This application note describes a method for two-channel simulated distillation by gas chromatography (GC) for both hydrocarbons and sulfur. The method utilizes a 7890A GC configured with a high-temperature programmable temperature vaporizer (HT-PTV) inlet and a sulfur chemiluminescent detector (SCD) mounted in series with a flame ionization detector (FID) by use of a special mounting adapter. A simulated distillation (SimDis) software program provides an easy-to-use solution for sulfur and hydrocarbon simulated distillation. The data show that observed boiling point (BP) values agree with the ASTM D2887 consensus BP values within the allowable differences. The system also demonstrates very good repeatability for both hydrocarbon and sulfur SimDis. An example of a light cycle oil (LCO) analyzed according to D2887 is also included.

This application note introduces the superior mass accuracy of the Agilent 6540 Accurate-Mass Q-TOF for high-confidence characterization of unknowns. The capability of the instrument was tested by analyzing a sample described as a compound with a nominal molecular weight of 218 and determining its correct molecular formula.

This application brief describes a highly repeatable method for the quantification of estradiol in human plasma using the Agilent 7000 Triple Quadrupole GC/MS. The method provides an LOD of 1 pg/mL (2 fg on-column) and RSDs for quantification of <15% in the 1 to 600 pg/mL concentration range.

This application note describes a rapid method for the quantification of total testosterone in serum using the Agilent 1200 Series Rapid Resolution LC (RRLC) system with the Agilent 6430 Triple Quadrupole LC/MS in atmospheric pressure chemical ionization (APCI) mode. A two dimensional LC approach was used to minimize matrix interferences and ion suppression, while reducing the time required for analysis. Excellent linearity of quantification (R2 > 0.99) was obtained in the range of 5 to 2000 ng/dL, with analysis times of only 4.5 minutes, and day-to-day precision was below 10%. The limit of detection (LOD) and limit of quantification (LOQ) were 2 ng/dL and 5 ng/dL respectively, in 5% bovine serum albumin.

This application note demonstrates a single quad GC/MS analysis of the GC/MS amenable listed allergens using the unique selectivity of an Agilent J&W DB-XLB capillary GC column

The performance of sequential stir bar sorptive extraction (sequential SBSE) in combi- nation with thermal desorption (TD)-GC/MS for screening of pesticide residues in water was described.

A method for the detection and quantification of 2,4,6-Trichloroanisole in wine at a concentration as low as 1 ppt was developed using the Agilent 7890A/7000 Series Triple Quadrupole GC/MS with a Pressure Controlled Tee configuration.

This Application Note describes how to use the Agilent 7700x ICP-MS with ISIS Discrete Sampling to perform high-throughput analysis of high-matrix samples. Using ISIS-DS, difficult, high-matrix analyses can be performed in a fraction of the time typically required, with no compromise in analytical performance.

This application note describes a method for determining the boiling point range of biodiesel (B100 and B20) using the Agilent 7890A Series GC systems.

A fast multielement simulated distillation method (SimDis) based on Low Thermal Mass (LTM) column technology is described.

The Dynamic MRM (DMRM) algorithm in the Agilent 6400 Series Triple Quadrupole LC/MS system automatically constructs DMRM timetables for multiple analytes throughout the LC/MS analysis based on the retention time window for each analyte. It allows the instrument to acquire MRM data only during a stated retention time window, thus reducing the number of concurrent ion transitions. In this study, 194 MRM transitions from a digest of complex protein standard was analyzed using DMRM and MRM modes in the Agilent 6410 Triple Quadrupole LC/MS system. Improved sensitivity, better peak symmetry, and up to ten-fold increase in the signal-to-noise (S/N) ratio are achieved using DMRM.