Agilent Manuals

Various modifiers were investigated regarding their influence on the characteristic mass and memory effect for boron determination by graphite furnace spectrometry. The best results were obtained using a zirconium nitrate-nickel nitrate mixed modifier, which resulted in high sensitivity (characteristic mass 200 pg), negligible memory effect, and increased tube lifetime (about 200 atomization cycles at 2800 °C). The method was successfully applied to determine boron in river water, drinking water, and sewage.

This application note describes the method for measuring chromium in environmental waters by atomic absorption spectrometry using a Zeeman corrected graphite tube atomizer. This method does not require the formation of a metal complex or extraction into an organic solvent for preconcentration, but directly injects the water sample into the graphite tube, which can reduce the possibility of sample preparation and contamination.

This paper describes a method for analyzing gold by atomic absorption. The method involves extracting gold with aqua regia and then analyzing the gold with flame atomic absorption spectrometry.

This document describes a method for monitoring antibody charge variants using Agilent 3100 OFFGEL fractionation and high sensitivity protein detection with the Agilent 2100 Bioanalyzer. This method can be used for quality control in the production of monoclonal antibodies in the biopharmaceutical industry.

This document introduces an automated MRM method optimizer for peptide protein quantitation. The MassHunter Optimizer software is used to optimize collision energies for 40 MRM transitions in LC/MS/MS analysis and compare them with collision energies from Q-TOF experiments. Optimized collision energies result in up to a two-fold increase in sensitivity and signal-to-noise ratio of MRM peaks. The MassHunter Optimizer software helps reduce the time and sample required for optimization by up to 200-fold.

This application note describes an onsite solution for detecting 13 volatile aromatic compounds in soil with the transportable Agilent 5975T LTM GC/MSD. The solution uses a manual SPME method for sample pretreatment. The transportability of the Agilent 5975T LTM GC/MSD makes onsite detection possible while saving soil sam- pling time and storage. The testing results proved that this solution is practical and effective for detection of volatile organic compounds in soil in the field. The lowest detection amount of this method is 1.0 µg/kg in soil.

The use of the forked platform graphite furnace atomic absorption spectrometry method for the determination of trace elements in stainless steel is described. This method has high sensitivity and low background interference.

describes the evaluation of a new forked platform design for the Agilent GTA graphite furnaces

The document describes the determination of Cd, Cr, Cu, Ni, and Pb in a concentrated CaCl2/NaCl solution using AAS. The analysis of such a high solids containing solution presents challenges, which are addressed through indirect flame atomization, direct flame atomization, and graphite furnace atomization methods. It is important to clean the nebulizer and measure a reagent blank during the analysis. The results are compared against a calibration graph. The document provides calibration data for Cd.

This method is applicable to a wide range of animal and plant tissues including dairy products, grains, fruit and vegetables and oil seeds such as poppy seeds. It is suitable for cadmium concentration in the range 0.01-0.3 µg/g. Sample is digested by wet ashing and cadmium is then complexed and extracted into an organic solvent. After removal of the spent aqueous phase the organo metallic complex is destroyed by the addition of dilute HNO3. This aqueous phase containing the stripped cadmium is then analyzed by Graphite Furnace Atomic Absorption Spectroscopy (GFAAS). The additional stripping step is employed to improve precision and facilitate the use of aqueous standards.

This study evaluated the performance of the Agilent 1260 Infinity Analytical SFC system for the analysis of thiourea in two model compounds. The results showed excellent linearity, repeatability, and limits of detection.

This document introduces a method for pesticide analysis using an Agilent J&W DB-35ms UI 20 m × 0.18 mm, 0.18 µm column, which can effectively separate organochlorine and organophosphorus pesticides with good separation and reproducibility.