Agilent Manuals

The present study developed a miRNA microarray analysis method suitable for tissue pathology applications. This method uses the miRNA Complete Labeling and Hyb Kit to extract miRNAs from FFPE samples, and then miRNAs are analyzed using a miRNA microarray. The results showed that this method can effectively extract miRNAs from FFPE samples and accurately analyze miRNA expression. This method provides a feasible method for miRNA analysis using FFPE samples.

An Application Kit for the Screening of Samples for Analytes of Forensic and Toxicological Interest using LC/QQQ MS/MS with a Dynamic MRM Transition Database

Extended ionization capability of thermal gradient focusing ESI in high-throughput in-vitro ADME assays

This article introduces a method for the high-pressure and high-flow analysis of polyaromatic hydrocarbons using the Agilent 1290 Infinity LC and Eclipse PAH columns.

This Application Note describes the development of a rapid resolution LC method for the separation of the Chinese herbal prescription Zuo Jin Pill into its six major con- stituents: magnoflorine, jatrorrhizine, coptisine, palmatine, berberine and evodiamine. The method uses an Agilent 1200 Series Rapid Resolution Liquid Chromatography (RRLC) system coupled with an Agilent 1200 Series Diode Array Detector (DAD). The separation was carried out on an Agilent ZORBAX Eclipse Plus C18 column (4.6 × 150 mm, 1.8 µm). The linear gradient was eluted with the mobile phase of an acetoni- trile – acetate buffer. All calibration curves showed good linearity (r2 >0.9998) within the test range 1.2-1200 µg/ml. The results demonstrate that this analytical method is simple, sensitive and reliable for rapidly analyzing six major bioactive compounds in ZJPs. It is useful for the comprehensive evaluation of the quality of this traditional Chinese Medicine (TCM).

This application note describes the method development options for separation of vitamin D2 and D3 by UHPLC. Partial separation of vitamin D2 and D3 was achieved by an isocratic LC method using a C18 column and methanol/acetonitrile mobile phase. Several optimization parameters were examined to increase resolution (Rs) including column selectivity and column length. A 2.1 mm × 150 mm Agilent ZORBAX StableBond-C18 column provided the best resolution. Further fine tuning included faster flow rates and mobile phase strength resulting in short analysis times of 3 to 1.5 minutes with Rs of 1.6 to 1.4. The performance of a 3.0 mm internal diameter (id) column was also compared to that of the 2.1 mm id column demonstrating higher sample loading before any loss of resolution in the closely spaced peaks.

A precolumn backflush system based on capillary columns using midpoint pressure control is described. Midpoint backflush is made possible with a Capillary Flow Technology (CFT) purged union controlled by an AUX EPC channel on the Agilent 7890A GC system. The key application discussed is prefractionation of crude oil that provides a high resolution separation of the C4 to C12 cut. A general backflush method using Polywax 500 is presented to illustrate the backflush concept.

This article describes a method for the analysis of phthalate content in childcare items and toys using GC/MS

This document introduces the method of SampliQ OPT solid phase extraction sorbent to clean up flavonoids in Ginkgo biloba tablets by HPLC-DAD.

The effects of extra-column volume on 50 and 150 mm columns in 2.1 and 4.6 mm internal diameters were made using a range of capillary tubing volumes installed between the autosampler and column. The results showed that, in using short columns, increases in tubing volume will reduce the column separation efficiency, while increases in tubing volume will have little effect on the separation efficiency of the long column, which is consistent with the results of other scholars.

This application note describes an approach for automating the biodiesel standard and sample preparation prior to GC analysis using the enhanced capabilities of the Agilent 7693A Automated Liquid Sampler (ALS).

This document presents a fast method development for salicylic acid process impurities using Agilent ZORBAX Rapid Resolution High Throughput Columns and Agilent 1200 Series Method Development Solution. The method was developed using ACD/AutoChrom software, allowing for screening of multiple columns, buffers, and organic solvents to find the best experimental conditions. The final result was an isocratic separation achieved in under 3 minutes.

High-Throughput LC/MS/MS for cytostatic drugs with an Agilent 1290 Infinity LC System coupled to a tandem mass spectrometer

Increased productivity by quick identification of samples that fail regulatory requirements using the Agilent 6140 single quadrupole LC/MS system and Analytical Studio Browser software