Agilent Manuals

This Application Note describes how a S9 human liver homogenate was used to simulate oxidative phase 1 metabolism of the beta-blocking drugs propranolol, metoprolol and pindolol. Afterwards, the samples were partially purified by precipitation/extraction with trifluoroacetic acid and hexane, followed by analysis with an Agilent 1100 Series LC/MSD trap XCT system. Fully automated LC/MS and LC/MSn analysis allowed for identification of the metabolites produced by the liver homogenate.

This document discusses the 1997 FDA ruling 21 CFR Part 11 and its impact on the pharmaceutical industry. It also describes the compliance features of the LC/MSD Trap Security Pack 1.0 software and the compliance services and training offered by Agilent Technologies.

In this Application Note, the Agilent 6140 quadrupole LC/MS and Agilent 1200 Series Rapid Resolution LC systems are combined to demonstrate the need for a fast-scanning, quadrupole MS detector, fast positive/negative switching, and uncompromised MS spectra quality in ultra-fast-scanning mode. The source of peak broadening of the MS peaks is explained and several application examples are given.

In this Application Note, the Agilent 6140 quadrupole MS, equipped with the multimode source, is combined with the Agilent 1200 Series Rapid Resolution LC system to develop a fast, generic LC/MS method for the analysis of 35 pharmaceutical compounds from 17 compound classes, reducing the method run time from 2.1 to 0.8 minutes.

Presents a flexible configuration of QuickSwap that allows use of larger id columns, pressure pulse injections, and variable column flow rates without having to change the restrictor or QuickSwap pressure.

This document introduces the ZORBAX Extend-C18 columns from Agilent Technologies, which can be used for separating explosive compounds. The columns offer various configurations for customized HPLC methods based on resolution, speed, and solvent usage. The document also highlights the advantages of different column configurations, including resolution, speed, and solvent savings. By simply substituting the column, users can choose the desired combination of resolution, speed, and solvent usage.

This Application Note describes an antibody-based method for identifying and counting blood cells from whole blood samples on the Agilent 2100 bioanalyzer without a washing step. The ability of the Agilent 2100 bioanalyzer to identify peripheral whole blood cells is demonstrated and compared to a flow-cytometry-based reference instrument.

This Application Note will demonstrate the use of the Agilent 1200 Series Rapid Resolution LC system with Rapid Resolution High Throughput (RRHT) columns for the separation of the ingredients found in a complex ginseng root extract. The information obtained with an ion trap MSn and MRM is used to determine the structure of the ingredients and for quality control purposes.

A fast, sensitive and reproducible technique for confirming the presence of drugs of abuse (DOA) in oral fluids (OF) using the Agilent G6410AA Triple Quadrupole Mass Spectrometer (QQQ) is presented.

This is an application note describing a method for quantification of digoxin using liquid chromatography-mass spectrometry (LC-MS) in human serum

The document discusses the challenge of analyzing minor impurities in partially decomposed samples using liquid chromatography/mass spectrometry (LC/MS) technique. It demonstrates the use of Agilent 1100 Series LC/MSD SL quadrupole mass spectrometer with ChemStation software to improve the detection of impurities. The experiment was conducted using a partially decomposed sample of the bronchodilator salbutamol. The application of data analysis and software tools enhanced the ability to detect impurities.

This application note presents the procedure for the analysis of morphine and its metabolites in blood plasma using electrospray LC/MS, a method which does not require derivatization.

This article compares the detection techniques UV, fluorescence, and mass spectrometry in combination with standard and capillary LC systems and columns with different internal diameters. The advantages and disadvantages of the different detector/column/LC system combinations are discussed.

Introduce a method for coupling nano-LC with MALDI mass spectrometry, which allows to deposit the LC run on the MALDI plate in real time, and thus couple the high resolution power of nano-RP-HPLC separation directly with MALDI mass spectrometry and decouple the aquisition of MS/MS from chromatographic separation and MS cycle time.

This document introduces the characteristics of the Agilent 1100 Series LC/MSD electrospray interface, including system composition, working principle, application range, etc.