Agilent Manuals

This Application Note describes the development of conventional HPLC and Rapid Resolution LC (RRLC) methods for the analysis of Buplerui (Chaihu). A comparison of fingerprint chromatograms of different Buplerui samples showed different peak profiles of different TCMs collected from different places. The method for the Agilent 1200 Series Evaporative Light Scattering Detector (ELSD) was optimized to achieve maximum response. A quantitative method was developed to determine the six types of saikosaponins in different TCMs and their preparations.

Agilent's Poroshell 300 reversed-phase HPLC columns offer higher resolution and flow rates for faster and more efficient separation of peptides, proteins, and antibodies.

This Application Note describes a simple, sensitive quantification method for lovastatin in human plasma, based on a liquid chromatography-electrospray ionization-tandem mass spectrometry (LC/ESI/MS/MS) technique. Solid phase extraction was used for the extraction of lovastatin from plasma, and simvastatin was used as the internal standard. For quantification, the triple-quadrupole MS was used in multiple reaction monitoring (MRM) mode. The monitored transitions were m/z 405.4 to 199.2 for lovastatin and m/z 419.1 to 199.2 for simvastatin. A linearity coefficient of R2 > 0.99 was observed over a wide range of 0.1 ppb to 50 ppb of lovastatin in human plasma. Several methods are currently available for the quantification of this statin, but sensitivity and shorter runtime were the major challenges in those methods. The advantages of the method described here are simplicity, short runtime (1.2 minutes) and better sensitivity through MRM.

This paper describes the use of gas chromatography coupled with triple quadrupole mass spectrometry (GC/MS/MS) to screen pesticides in Traditional Chinese Medicine (TCM) at levels as low as 1 ppb. The Triple Quadrupole mass spec- trometer, when used in Multiple Reaction Monitoring (MRM) mode, can dramatical- ly remove matrix interferences and significantly increase the effective signal-to- noise ratio (S/N).

This article introduces the characterization of lavender oil using Agilent J&W DB-1ms Ultra Inert capillary GC columns. Gas phase analytical techniques including GC/MS, GC x GC, and GC/TOF were used to analyze the main components in lavender oil samples from several suppliers. The well-resolved components were then identified using a single quad GC/MS versus NIST Library 5.01, and the identified peaks served as the fingerprint for analysis. The same separation using GC-FID could be used to evaluate subsequent samples quickly and cost effectively. The well-resolved, sharp peaks observed on the Agilent J&W DB-1ms Ultra Inert column made this approach possible.

This document introduces the Agilent BenchCel Workstations Automated Microplate Centrifugation Solution, which can be used for PCR purification, cell harvesting, post-PCR process condensate spin-down, high density plate air bubble removal and many more applications. This solution integrates a wide range of instruments with the Agilent BenchCel Microplate Handler to create flexible benchtop automation solutions.

This document describes the features and benefits of the Agilent BenchCel Workstations Automated Barcode Labeling Workstation Application Bulletin. The workstation offers high-speed plate transfers and labeling, scalability to integrate additional devices, and storage options for up to 320 microplates.

This document describes the applications of Agilent BenchCel Workstations Microplate Sealing Solution, including compound storage, screening, PCR/qPCR/rtPCR, and sequencing. An Automated Microplate Sealing Workstation can be created by integrating the BenchCel Microplate Handler and the Agilent PlateLoc Thermal Microplate Sealer, providing high throughput and easy operation.

This application note describes the characterization of a monoclonal antibody at low nanogram levels using an Agilent HPLC-Chip system coupled to an Agilent Accurate-Mass Q-TOF MS instrument. The superior sensitivity and mass accuracy of the HPLC-Chip LC/MS platform, combined with the powerful data processing capabilities of Agilent MassHunter and BioConfirm software, enabled easy and rapid identifi cation of antibody heterogeneity and cleaved fragments. Robust, reliable, and easy to use, Agilent nanofl ow LC/MS technology is ideally suited for the routine analysis of biopharmaceuticals.

This document introduces the Agilent gDNA Isolation Workstation, which can efficiently extract DNA and can process up to 10 microplates at a time. The processing time for each microplate is approximately 10 minutes.

The Agilent PCR Preparation Workstation is a flexible workstation that provides high-throughput PCR preparation. Up to 150 microplates can be processed in one run (with tip changing). The plate processing time is approximately 5 hours for a 50 microplate run, depending on the protocol complexity.

This document describes the application of the Agilent Cell Viability Workstation and Promega CellTiter-Glo kit. The Agilent Cell Viability Workstation consists of a Molecular Devices SpectraMax M5 and an Agilent BenchCel Microplate Handling Workstation integrated with an Agilent Bravo Automated Liquid Handling Platform. This workstation is designed for automated cell viability testing and features a compact footprint and high-throughput processing capability. The Promega CellTiter-Glo kit utilizes a linear relationship between ATP concentration and light signal, measuring the ATP content in cells through the generation of luminescence to determine the number of viable cells.

Analysis of Aminoglycoside Antibiotics

This article introduces the application of the Agilent Technologies 6140 Single Quadrupole LC/MS High Throughput Mass Spectrometer. The Agilent 1200 Series Rapid Resolution Liquid Chromatography (RRLC) system is used to analyze the impurities of octyl dimethyl para-aminobenzoic acid (OD-PABA). The instrument has a high scan speed capability, which allows clear detection of the degradation product p-dimethylbenzoic acid. The background and usage of OD-PABA are also discussed in this article.