Agilent Manuals

This document introduces the key technology in protein analysis-size exclusion chromatography (SEC), and illustrates the principle, characteristics and application of SEC with Agilent ProSEC 300S column as an example

This note describes the analysis of albumin proteins using Agilent ProSEC 300S columns. Size exclusion chromatography (SEC) is used to investigate the aggregation of albumin proteins in solution, including dimers and trimers. This technique is of interest in biomedical applications.

This document describes a method for the rapid, sensitive and reproducible detection of seven common drugs of abuse using the Agilent 500 Ion Trap LC/MS.

This application note describes a rapid and sensitive LC/MS/MS method for the detection and analysis of four aflatoxins (B1, B2, G1, and G2) that provides detection down to 1 ppb. The 500 Ion Trap MS/MS capabilities result in the collection of clear, baseline resolved chromatographic peaks for target compound quantitation.

This document describes the analysis of drugs of abuse using GC/MS with the Ultra Inert Inlet Liners with Wool. These liners provide excellent inertness, homogeneous sample mixing and evaporation, non-volatile residue trapping, and column and detector protection for drugs of abuse screening.

This document presents the preliminary results of a collection on an unusual macromolecular RNA G-quadruplex structure. The single crystal data was collected to 2.2Å resolution, providing us with a detailed view of the RNA’s sugar pucker and hydration structure. The efficient data collection geometry and associated high redundancy of data has enabled us to also visualize the anomalous scattering from the ions in the central channel, and confirm the location of the bromine atoms included to assist in phasing.

The Ultra Inert liners with wool provide superior inertness for accurate analysis of semivolatile analytes by GC/FID, homogeneous sample mixing and evaporation, and maximum column and detector protection.

This document describes a high-temperature simulated distillation method for analyzing heavy crude oils and petroleum blends. The method uses a new metal column that has been deactivated by a proprietary process to provide excellent performance and exceptional durability at high temperatures.

Two methods have been developed on the Agilent 7000 Triple Quadrupole GC/MS system for the analysis of polychlorinated biphenyl (PCB) congeners in foodstuffs and animal feed. The methods were shown to give linear response over the required concentration ranges. In addition, quantitative results for dioxin-like PCB (dl-PCB) congeners down to low pg TEQ/g levels and non-dioxin-like PCB (ndl-PCB) congeners at levels below 1 ng/g product were in good agreement with values obtained using a GC-High Resolution mass spectrometer. This application note demonstrates the determination of the 12 dl-PCB comprising eight mono-ortho PCB congeners (# 105, 114, 118, 123, 156, 157, 167 and 189) and four non-ortho PCB congeners (# 77, 81, 126 and 169) as well as the six ndl-PCB congeners (# 28, 52, 101, 138, 153 and 180) that are also known as 'Indicator PCB' congeners.

This document describes the extraction and analysis method of marijuana and its metabolites in human blood, as well as the use of related instruments and reagents.

This article describes a method for analyzing the concentration of FAME and TG in biodiesel fuel using the Agilent 1200 Series HPLC system. Biodiesel is a motor or heating fuel produced from renewable vegetable oils or animal fats. It is produced by reacting vegetable oil with methanol in the presence of a catalyst, resulting in a mixture of fatty acid methyl esters (FAME) and glycerin. After removing glycerin and other contaminants, the remaining FAME mixture is pure biodiesel. Depending on the oil source, a typical biodiesel contains FAME mixtures with both saturated and unsaturated carbon chains from C8 to C24. This application note demonstrates an exemplary analysis of the concentration of FAME and triglycerides (TG) in biodiesel fuel (diesel oil).

This note shows how the Agilent PLgel 5 µm gel permeation chromatography column matches the performance of another leading column, even though it has slightly different dimensions.

A rapid method was developed on the Agilent 7000 Series Triple Quadrupole GC/MS system to screen for more than 150 doping agents in seven classes of substances, at or below WADA MRPLs

This document introduces the features and usage of Varian's VS series vacuum furnace leak detector.