Agilent Manuals

This article introduces a method for studying the negative thermal expansion of metal cyanides using variable temperature powder diffraction.

This document presents the application of GPC/SEC for the analysis of polyolefins. It covers the GPC separation mechanism, system requirements for polyolefin analysis, sample preparation, system, software and standards, as well as GPC analysis of different types of polyolefins.

This application note introduces novel statistical methods for determining the absolute structure of light-atom organic molecules. All of these methods require precise experimental determination of very small differences in the intensities of related reflections, which is facilitated by Agilent’s micro-focus SuperNova X-ray diffractometer.

Two samples consisting of various nonsteroidal anti-inflammatory drugs (NSAID) are separated using methods developed on an Agilent 1200 Series Method Development Solution controlled by ACD/Labs AutoChrom for ChemStation software. The chromatograph while under control of AutoChrom can screen up to seven columns, thirteen buffers and two organic solvents with columns held in four temperature controlled zones. The software helps plan the next best experiment to perform, allowing the analyst to focus on quickly developing methods using conditions with the best likelihood of success. In this work four columns (Agilent ZORBAX StableBond SB-C18, Agilent ZORBAX Eclipse Plus C18, Agilent ZORBAX Bonus RP and Agilent ZORBAX Eclipse Plus Phenyl-Hexyl) are screened using five mobile phase modifiers. Twenty solvent column experiments are initially screened for each sample set.

This application note demonstrates the capabilities of the Agilent J&W CP-COX column with the Agilent 490 Micro GC, including separation of permanent gases and backflush possibilities to ensure extended column lifetimes.

Agilent Bravo high-throughput protein purification system and AssayMAP1 protein A cartridges can efficiently purify human IgG

This Application Note describes the development of an immunoprecipitation method in combination with microfluidic on-chip analysis on the Agilent 2100 Bioanalyzer for sensitive, selective and quantitative detection of endogenous β-galactosidase in crude E. coli lysates. The effects of antibody selection and the importance of preclearing steps for sensitive and specific detection of the target protein are discussed. Quantification of β-galactosidase was achieved via an external calibration curve based on various spiking levels of pure β-galactosidase into E. coli lysate. Results proved the described approach to be quantitative over orders of magnitudes and highly reproducible.

This document describes the use of the Agilent 1260 Bio-inert Quaternary LC system for sensitive and rapid LC methods to characterize the therapeutic protein, P128. Optimized peptide maps were constructed using sub-2-µm column technology to decrease analysis speed for this protein. Size exclusion chromatography (SEC) and ion exchange chromatography (IEX) were performed using the new Agilent Bio-HPLC columns to detect potential impurities or isoforms of the protein preparation. The bio-inertness and corrosion resistance of the instrument coupled with the simple and reproducible methods make this solution suitable for routine use in QA/QC labs.

This Application Note introduces the concept of peak capacity and investigates the effects of gradient parameters such as flow rate, gradient time, column length and gradient slope on peak capacity. The results show that flow rate and gradient time have a significant effect on peak capacity. The effects of column length and gradient slope on peak capacity are relatively small.

This application note investigates the effects of gradient parameters on peak capacity and provides guidance on maximizing peak capacity by adjusting parameters. The study utilizes the Agilent 1290 Infinity LC system, known for its wide power range and high column backpressure tolerance. The variation of peak capacity values with flow rate, gradient time, column length, and slope of the gradient is discussed.

This Application Note describes a method for simultaneous determination of eight FDA/EP approved active UV filter ingredients in under two minutes using the Agilent 1290 Infinity LC and Agilent Poroshell columns. The method offers short analysis time, high sensitivity, robustness and good resolution, and is suitable for qualitative and quantitative analysis of active UV filters in commercial personal care products.

This document describes the GC analysis of sulfur components in propylene using a Pulsed Flame Photometric Detector (PFPD). The analysis method allows for the measurement of trace levels of target components in C3 hydrocarbon streams without any matrix interference.

This document is about the use of the Agilent 7696A Sample Prep WorkBench, which describes how to use the tool to automate the serial dilution of samples and measure the performance of flame ionization detectors.

The Agilent 1260 Infinity Method Development Solution is an automated solution for method development that allows for automatic scaling of gradient times and flow rates for different column lengths and diameters. It utilizes the Agilent ChemStation Method Scouting Wizard to automate the setup of method development sequences and provides all needed methods for column scouting, variation of mobile phases, gradients, and temperatures.

This application note presents a method for the analysis of adulterants in weight loss supplements marketed as “all natural.” The analysis is performed using the Agilent 500 Ion Trap LC/MS with Turbo Data Dependent Scanning (TurboDDS), which performs full scan and MS/MS analysis in a single injection. Target adulter- ants were observed and yielded spectra with excellent library matches to the NIST MS Library when spiked into a dietary supplement matrix at 10 µg/mL and then diluted 1:10 in water. A real incurred weight loss product was also analyzed and found to be adulterated with Bumetanide. Unlike other techniques that use targeted analysis to detect known adulterants in a dietary supplement of interest, TurboDDS screens and identifi es both known and unanticipated adulterants in a single experiment.