Agilent Manuals

This document highlights the method and advantages of using the Agilent Cary 60 UV-Vis spectrophotometer for determining drug tablet concentration in pharmaceutical applications.

The application note demonstrates how the Cary 60 is used to measure the purity of DNA at 4 °C using the micro fiber optics accessory – resulting in significant time and cost savings without compromise in the accuracy and reproducibility of data quality.

This application note details a quick and effective analytical method for the determi- nation of low ppm and trace level organophosphorus (OP) pesticide residues in an olive oil extract.

This application note presents a method for separating peptides using acetonitrile-free gradient elution on SepTech ST150 10-C18. In response to the global shortage of acetonitrile, alternative solvents such as ethanol have been investigated, and the performance of SepTech ST150 10-C18 is compared to three leading competitor materials. The results demonstrate the superior resolution of peptides achieved by SepTech ST150 10-C18 under acetonitrile-free mobile phase conditions.

Fast (5 min), dilute-and-shoot method for the determination of morphine and one of its major metabolites (morphine-glucuronide) in biological fluids (saliva and urine) using the Agilent 500 Ion Trap LC/MS

This application note describes the advantages of using Agilent PLgel 10 µm MIXED-B columns for high MW polymer analysis under demanding eluent conditions such as high temperature gel permeation chromatography (GPC).

This document describes how to use Agilent PLgel 5 µm MIXED-D columns to analyze polyaniline.

This application note provides detailed information on how to use Agilent PL-GPC 50 Plus to perform GPC with viscometry on starches. The column, mobile phase, detectors, and other configurations are introduced. The separation results of two starch samples are also analyzed.

This application compendium provides information on using high performance liquid chromatography/size exclusion chromatography (GPC/SEC) to analyze biodegradable polymers.

This note describes an MSn data acquisition method using the 500 Ion Trap LC/MS TurboDDS software for the analysis of 7-ethoxycoumarin and two of its metabolites. TurboDDS saves time and streamlines data reporting for multiple stages of MS/MS and MSn analys.

The performance of the Agilent 500 Ion Trap LC/MS in EPA Method 535 is evaluated in terms of calibration and quantitative accuracy in a variety of matrices. The data reveals this instrument provides reliable quantitative data, even with the presence of highly concentrated surface water extracts.

This document describes the extraction of polar basic drugs from plasma using polymeric solid phase extraction cation exchange technique. The technique allows for rapid and efficient removal of neutral and acidic interferences, as well as concentration of basic analytes, resulting in improved analytical performance and sensitivity in the quantification of basic compounds.

This report describes a method by which a crude sample of solid phase synthesized leuprolide can be purified on a PLRP-S 100Å 10 μm column.

The document describes the process of preparative scale purification of a sample of synthetic bradykinin using a concentration overload regime.

This application note describes the use of Agilent PLRP-S 100Å reversed phase media for the purification of synthetic acyl-carrier protein (ACP) fragment 65-74.