This document introduces the working principle of Agilent 1200 Series Evaporative Light Scattering Detector (ELSD) and provides a method for using mobile phase compensation to significantly improve the quantification of unknowns.
The document explains that the Agilent 1200 Series Evaporative Light Scattering Detector (ELSD) can quantify compounds without available standards but that gradient runs may cause errors due to differences in organic content between standards and unknowns. It introduces a mobile phase compensation method that uses a separate pump attached post-column to run an opposite gradient, neutralizing the changing organic content and creating an isocratic flow to the detector.
| Publication Number | 5990-4275EN |
|---|---|
| Publication Date | July 1, 2009 |
| Software Version | B.01.01 |
| T connector Part Number | 5022-2144 |
| Red tubing Part Number | 5042-6461 |
| ELSD Part Number | G4218A |
| Binary Pump Part Number | G1312B |
It compensates for changing organic content in gradient elution by using a separate pump to run an opposite gradient, which neutralizes the effect and allows for isocratic flow to the detector.
The second pump is attached post-column to run an opposite gradient that neutralizes the effect of changing organic content, ensuring an isocratic flow reaches the detector.
The pumps are matched by adjusting the start time of the pumps so that they produce exactly opposite gradients at the "T" connector.