This note provides recommendations for the use of extremely short effective length capillaries in CE. It discusses the advantages of using very short capillaries (less than 10 cm) and the limitations that must be considered when using extremely short capillaries in a CE instrument. The note also introduces methods of injecting samples into extremely short capillaries, including injection from the outlet end of the capillary and injection from the inlet end of the capillary.
This document is a technical note explaining how using extremely short effective lengths (below 10 cm) in capillary electrophoresis can significantly reduce analysis times while maintaining or improving peak efficiency and sensitivity. It details the advantages of this approach for simple mixtures, initial screening, and method development.
The minimum effective length can be 8.5 cm by injecting the sample from the outlet end of the capillary.
Using an 8.5-cm effective length reduces the analysis time to about two minutes.