This report introduces the application of Agilent's SampliQ C8 and Amino SPE tubes. This method can detect the presence of four steroids in urine. These four steroids can be used as growth promoters for food-producing livestock. However, if used improperly, they can be harmful to human health.
This document details a sample preparation method for detecting illegal anabolic steroid use in livestock by analyzing urine samples. The procedure involves enzymatic hydrolysis followed by solid phase extraction (SPE) cleanup using Agilent's SampliQ C8 and Amino SPE tubes to isolate and purify free steroids from the matrix.
The study tested four representative anabolic steroids: prednisolone, dexamethasone, 1,4-androstadiene-3,17-dione, and norgestrel. The combined use of the C8 and Amino tubes provided high recoveries (95% to 99%) with excellent reproducibility (RSDs of 1.0% or lower), resulting in a cleaner sample suitable for HPLC analysis.
The recovery for norgestrel was 86%.
The four representative anabolic steroids used were prednisolone, dexamethasone, 1,4-androstadiene-3,17-dione and norgestrel.