This document describes the performance characteristics of the Agilent 1290 Infinity Autosampler, including the highest precision and the lowest carryover and fastest cycle times.
The document describes the Agilent 1290 Infinity Autosampler, which utilizes a flow-through or variable loop design to ensure precise injections of volumes ranging from 0.1 pL to 20 pL (optionally 40 pL) without hardware changes. The design features a reduced hydraulic volume of 80 µL and includes Peltier temperature control from 4 to 40 °C for thermally-labile samples.
Performance evaluations demonstrate area precision below 0.161% RSD for conventional isocratic runs and below 0.56% RSD for ultrafast gradient conditions at 0.5 pL injection volumes. The autosampler achieves a carryover of less than 0.003% using an exterior needle wash routine, and supports a minimum accessible sample volume of 1 pL extracted from a 3 pL sample volume using conical 100-pL vials.
| Injection volume range | 0.1 to 20 pL (40 pL optional) |
|---|---|
| Temperature control range | 4 to 40 °C |
| Carryover | < 0.003% |
| Minimum accessible sample volume | 1 pL from 3 pL |
The autosampler can inject precisely over a range of 0.1 pL to 20 pL, or optionally up to 40 pL.
It features a new cartridge loop design with Peltier temperature control from 4 to 40 °C.
The carryover is typically less than 0.004% for chlorhexidine when using the exterior needle wash routine.