Varian ELSD Advantage Statement: The high sensitivity of the Varian Evaporative Light Scattering Detector is evident from the large response it delivers, even with low column loading. This note describes the calibration of the Varian ELSD response with a GPC application.
This note details the calibration of the Varian Evaporative Light Scattering Detector (ELSD) response within a Gel Permeation Chromatography (GPC) application. The procedure involves determining the detector's response for specific calibration solutions to ensure accurate measurements.
The test utilized a polystyrene narrow standard (Mp 30,300) eluting through a PLgel MIXED-D column with a fixed injection volume of 100 µL. A series of calibration solutions ranging from 1 to 40 ng of column loading were prepared by diluting a stock solution. The results demonstrated that the ELSD provides a linear response to column loading, even at low concentrations.