LC/MS columns have outstanding separation and fast equilibration time in short columns, small particles (3.5 vs. 5 µm), gradient mode.
The document describes a method to reduce analysis time by utilizing shorter columns with smaller particles (3.5 vs. 5 um) and low-volume cartridge columns. It highlights that gradient methods work well with these columns, offering good chromatography and fast equilibration times.
A specific example is provided where four compounds are resolved in less than one minute using a 0 to 100% gradient over 45 seconds of re-equilibration time. The separation can be repeated in under two minutes thanks to the small column volume and high flow rate.
The analysis uses a flow rate of 4.0 mL/min.
Re-equilibration takes only 45 seconds.
Narrow peak widths of only 0.02 to 0.05 min. are common.