This application note describes how to use Agilent ZORBAX Rapid Resolution High Throuhput (RRHT) Eclipse Plus Phenyl-Hexyl column for high-resolution analysis of 13 taxane derivatives. Improved silica, endcapping, and bonding technology, as well as the molecular interactions of the phenyl-hexyl moiety with the conjugation within the taxane derivatives, make the Eclipse Plus Phenyl-Hexyl column an ideal choice for this RRHT taxane method.
This document details a chromatographic method for separating thirteen taxane derivatives, including paclitaxel and docetaxel, using an Agilent ZORBAX Eclipse Plus Phenyl-Hexyl column. The study demonstrates that this column provides superior resolution compared to C18 columns for these complex diterpene structures.
The method utilizes an Agilent 1200 Rapid Resolution LC (RRLC) system with a gradient elution of 0.1% formic acid in water and acetonitrile. Results show that high resolution is maintained even when a Single Quadrupole Mass Spectrometer is installed in series after the diode array detector, making it suitable for drug discovery and analysis of cancer treatment compounds.
| Column Dimensions | 4.6 x 50 mm |
|---|---|
| Particle Size | 1.8 um |
| Part Number | 959941-912 |
| Flow Rate | 1 mL/min |
| Injection Volume | 5.0 uL |
| Column Temperature | 25 °C |
| Detection Wavelength | 254 nm |
| Maximum Pressure | approximately 240 bar |
The Agilent ZORBAX Eclipse Plus Phenyl-Hexyl column is recommended as it provides excellent separation and better selectivity than C18 columns.
Yes, no significant loss in chromatographic resolution is noted when an Agilent G1956B Single Quadrupole Mass Spectrometer is installed in series.
The maximum pressure reached during this analysis was approximately 240 bar with the pulse damper used.