This article describes how to use Agilent ZORBAX Eclipse Plus Phenyl-Hexyl and StableBond Phenyl columns to separate potentially harmful azo dye amines
The document details a method for resolving potentially harmful aromatic amines that originate from azo colorants. These amines are suspected carcinogens often found in consumer goods like textiles and cosmetics, where they are banned by EU Directive 2002/61/EC.
Two specific Agilent ZORBAX phenyl columns were utilized to achieve separation: the ZORBAX Eclipse Plus Phenyl-Hexyl column and the ZORBAX StableBond Phenyl column. The text explains that these two phases possess distinct chemical differences, such as endcapping and hydrocarbon content, which provide significantly different selectivity factors for aromatic amines containing phenyl groups.
Mobile phase A is 10 mM ammonium acetate in water, pH 4.7 with acetic acid; Mobile phase B is methanol.
The two phenyl phases have distinct chemical differences that provide significantly different selectivity to confirm identification of banned amines.