This application note describes a method for rapid analytical separation and quantification of human polyclonal IgG using the Agilent Bio-Monolith Protein A HPLC column. The method is applicable to the separation of IgG from complex mixtures and can also be used to monitor fermentation titers throughout large scale IgG production.
The document presents a method for the analytical separation and quantification of polyclonal IgG from complex mixtures, such as human plasma or cell culture supernatants. It highlights that the Bio-Monolith Protein A column offers high selectivity and recovery with flow rate-independent separations due to its continuous channel monolithic structure.
| Column ID x Length | 5.2 mm id x 4.95 mm |
|---|---|
| Part Number | 5069-3639 |
| Detection Wavelength | 280 nm |
| Flow Rate | 1 mL/min |
| IgG Recovery | 95 to 100 percent |
IgG elutes in approximately 1.5 minutes.
Yes, the method can be used to monitor fermentation titers throughout large scale IgG production.