Agilent 1100 Series Analysis of Bendiocarb Metabolite by HPLC

This document describes the HPLC analysis method of bendiocarb and metabolite

Brand: Agilent
Category: Other
Document type: Application Note
Language: English
Pages: 2
File format: PDF
File size: 151 KB
Published: 02 July, 2012
Updated: 01 October, 2023
Model: 1100 Series
MD5 checksum: AADF45B978053626D5DD0E51EC6A4EC6
PDF: Agilent 1100 Series Analysis of Bendiocarb Metabolite by HPLC PDF

About this manual

This document details a High-Performance Liquid Chromatography (HPLC) method for the analysis of the insecticide bendiocarb and its metabolite. The method utilizes a Hypersil ODS C18 column with a mobile phase of water and acetonitrile, detected at 212 nm using a diode array detector. The procedure covers extraction from soil and water, as well as separation and detection parameters.

What's inside

Abstract
Separation
UV-visible detection
Conditions
Sample preparation
Equipment

Specifications

Column	100 x 2.1-mm Hypersil ODS C18, 5 ym
Mobile phase	Water—acetonitrile (65:35 isocratic mixture)
Flow rate	0.36 ml/min
Temperature	40 °C
Detection wavelength	212 nm (16 nm bandwidth)
Detection limit	4 yg/!

Frequently asked questions

What equipment is used for sample preparation?

Samples can be extracted from soil using Soxhlet equipment or by ultrasonic treatment in solution, and from water using liquid—solid or liquid-liquid techniques.

What is the detection limit for this method?

The detection limit is 4 yg/! without sample enrichment.

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