This document describes method parameters specific for the Agilent system equipped with DAD detection and computer control through Agilent ChemStation.
Agilent Technologies provides recommended operating conditions for its Capillary Electrophoresis system to satisfy the requirements of a new ASTM standard for determining dissolved inorganic anions in aqueous matrices. The document specifies parameters for the system equipped with DAD detection and computer control via Agilent ChemStation, including cassette temperature, polarity, voltage limits, and current settings.
| Cassette temperature | 25°C |
|---|---|
| Polarity | negative |
| Capillary length (L) | 64.5 cm |
| Flush buffer volume | 1 mL |
| Waste vial volume | 0.6 mL |
| Run buffer replacement interval | 10 runs |
| Sample dilution factor | 1:20 |
| Filter pore size | 0.45 µm |
The recommended parameters include a cassette temperature of 25°C, negative polarity, and specific injection and detection settings.
A new capillary is prepared by flushing 0.5 N NaOH for 5 min, water for 1 min, and run buffer for 3 min at 1 bar.
Waste water samples should be diluted 1:20 and filtered through a 0.45 µm filter prior to injection.