Agilent Extended Ionization Capability of Thermal Gradient Focusing ESI Application Note

About this manual

The document describes a method using an Agilent 1290 Infinity UHPLC system coupled with an Agilent 6460 Triple Quadrupole mass spectrometer featuring thermal gradient focusing ESI (AJS). This setup was utilized to streamline high-throughput bioanalytical method development for in-vitro metabolic stability samples. The study compared AJS technology against conventional orthogonal ESI using generic source values.

What's inside

• Extended ionization capability
• Thermal gradient focusing ESI (AJS)
• High-throughput in-vitro ADME assays
• Metabolic stability samples
• Comparison with conventional ESI

Frequently asked questions

What is the maximum flow rate allowed by the Agilent 1290 Infinity LC Triple Quadrupole MS/MS system?

The system allows flow rates up to 2 mL/min.

What is the maximum pressure limit of the Agilent 1290 Infinity LC Triple Quadrupole MS/MS system?

The system supports pressures up to 1200 bar.

What is the minimum dwell time achievable with this system configuration?

Dwell times as low as 1-2 ms are possible.