This document presents a fast method development for salicylic acid process impurities using Agilent ZORBAX Rapid Resolution High Throughput Columns and Agilent 1200 Series Method Development Solution. The method was developed using ACD/AutoChrom software, allowing for screening of multiple columns, buffers, and organic solvents to find the best experimental conditions. The final result was an isocratic separation achieved in under 3 minutes.
The document details a study where salicylic acid and its published process impurities were separated using an Agilent 1200 Series LC system. The method development was automated and controlled by ACD/AutoChrom software version 12.01, which allowed for the screening of multiple columns, buffers, and solvents to quickly identify optimal separation conditions.
The G1312B Binary Pump SL used in the system is capable of delivering up to 600 bar.
The temperature was maintained at 25 °C throughout the experiment.
Good response for all compounds was found at a 230 nm detection wavelength.