This document introduces the method of SampliQ OPT solid phase extraction sorbent to clean up flavonoids in Ginkgo biloba tablets by HPLC-DAD.
This document presents a validated analytical method for the cleanup of flavonoids (quercetin, kaempferol, and isorhamnetin) from Ginkgo biloba tablets using Agilent SampliQ OPT Solid Phase Extraction (SPE) sorbents. The study demonstrates that this technique significantly reduces matrix interferences compared to untreated samples.
| Column dimensions | 4.6 mm x 75 mm, 3.5 µm |
|---|---|
| Flow rate | 1 mL/min |
| Injection volume | 3 µL |
| Column temperature | 35°C |
| Mobile Phase A composition | 0.5% Phosphoric acid, pH 2.7 |
| Mobile Phase B composition | Methanol |
| Isocratic ratio | 40% A 60% B |
| Detection wavelength | 370 nm |
The optimized procedure involves conditioning with methanol, equilibrating with water, loading the dilute sample, washing twice (once with a methanol/water mix and once with ammonium acetate), and eluting with methanol.