This document introduces a rapid method for monitoring carbohydrates in food using capillary electrophoresis with indirect UV detection. The method is simple and stable and is suitable for application in routine laboratories.
This document presents a method for the rapid monitoring of carbohydrates in processed foods, such as fruit juices and yogurt, to ensure regulatory compliance. The technique utilizes capillary electrophoresis with indirect UV-detection, offering a simpler and more stable alternative to high-performance liquid chromatography (HPLC) for routine laboratory analysis. The method is particularly suitable for complex sample matrices where classical stationary phases may present challenges.
The procedure involves the use of an Agilent Capillary Electrophoresis system equipped with DAD detection and ChemStation software. Key components include the Agilent Basic Anion Buffer and a bare fused silica capillary. The method demonstrates linear results over a range of 50-10,000 ppm with detection limits between 7-15 ppm, successfully analyzing sucrose, fructose, glucose, and lactose in various food samples.
The document describes a method for the rapid monitoring of carbohydrates in food using capillary electrophoresis with indirect UV-detection.
The method detection limits are 7-15 ppm.
The analysis is performed at a voltage of -25 kV.