Agilent 1100 Series LC/MS Analysis of Natural Food Colorants Application Note

The document describes the features and characteristics of a product, including detailed product specifications and function descriptions.


Agilent 1100 Series LC/MS Analysis of Natural Food Colorants Application Note - cover page
Brand
Agilent
Category
Other Electronics
Document type
Application Note
Language
English
Pages
9
File format
PDF
File size
903 KB
Published
12 June, 2012
Updated
28 September, 2023
MD5 checksum
1449ED31AEE26491E2A09B516E26E530

About this manual

This document details the analysis of natural food colorants found in beverages, jellies, and candies. It focuses on developing reliable analytical methods for red cabbage, paprika, Monascus, and lac colorants using Liquid Chromatography/Mass Spectrometry (LC/MS).

The study utilizes an Agilent 1100 Series binary pump system coupled with an LC/MSD. The document provides specific experimental conditions, including mobile phases, gradient profiles, and MS parameters for both Electrospray Ionization (ESI) and Atmospheric Pressure Chemical Ionization (APCI) sources to identify major pigments in each sample.

What's inside

  • Introduction
  • Experimental
  • Red cabbage colorant
  • Monascus colorant
  • Paprika colorant
  • Lac colorant
  • LC conditions
  • MS Conditions
  • Conclusion

Specifications

SystemAgilent 1100 Series binary pump, thermostatted column compartment, vacuum degasser, autosampler, and LC/MSD
Flow rate0.2 ml/min
Column temp40°C
Injection vol10 ul
Vcap voltage4000 V
Scan range100-1200 amu

Frequently asked questions

Which natural colorants were analyzed in this study?

The study analyzed four natural colorants: red cabbage, paprika, Monascus, and lac.

What is the common fragment ion observed in the mass spectra of paprika colorant pigments?

A common fragment ion was observed at m/z 567 in the mass spectra of these pigments.

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