This document introduces the method for detecting fumonisins using Agilent 1100 Series LC/MSD without derivatization.
The document details a method for detecting fumonisins, toxic compounds produced by the fungus Fusarium found in corn. It highlights that traditional HPLC methods often require derivatization, whereas this specific application demonstrates detection using the Agilent 1100 Series LC/MSD with electrospray ionization (ESI) and collision-induced dissociation (CID).
| Column dimensions | 150 x 2.1 mm |
|---|---|
| Mobile phase A composition | 5 mM ammonium acetate in water, pH 3 |
| Mobile phase B composition | acetonitrile |
| Column temperature | 40°C |
| Ionization source | ESI |
| Ion mode | Positive |
No, the Agilent 1100 Series LC/MSD can detect fumonisins without derivatization.
The analysis uses positive ion mode.