This paper introduces a method for detecting vitamins using high performance liquid chromatography (HPLC). This method can simultaneously detect fat-soluble vitamins and water-soluble vitamins. The method has high sensitivity, selectivity, and can simultaneously detect multiple vitamins.
The document details a High-Performance Liquid Chromatography (HPLC) method for the analysis of both fat-soluble vitamins (A, D, E) and water-soluble vitamins (B1, B2, B3, B6, B9, B12, C). It emphasizes the necessity of identification alongside quantification due to interfering compounds with similar chemical properties. The method utilizes UV-visible diode-array detection or electrochemical detection at room temperature.
| Column | 100 x 4mm Hypersil BDS, 3 um |
|---|---|
| Mobile phase A | water with pH = 2.1 (H2SO4) = 99% |
| Mobile phase B | ACN + 10% A+ 1 % |
| Flow rate | 0.5 ml/min |
| Column compartment temperature | 30 °C |
| Injection volume | 2—5 ul |
| Detector wavelength | UV-DAD detection wavelength 220/30 nm |
| Limit of detection | <500 pg (injected amount), S/N = 2 |
The method uses an Agilent 1100 Series system including a vacuum degasser, quaternary pump, autosampler, thermostatted column compartment, and diode array detector.
The system requires Agilent ChemStation software.