Analysis of amino acids in beer using HPLC with online derivatization.
The document details a High-Performance Liquid Chromatography (HPLC) method for analyzing both primary and secondary amino acids in beer samples. The procedure utilizes automated online precolumn derivatization followed by fluorescence detection to ensure short analysis times and simple sample preparation.
Specific chromatographic conditions are provided, including the use of a Hypersil ODS column with a specific gradient mobile phase. The method achieves a limit of detection of 5 pmol and demonstrates high repeatability, making it suitable for detecting potentially toxic amino acids or determining protein origins.
| Limit of detection | 5 pmol |
|---|---|
| Repeatability of RT over 6 runs | <1% |
| Linearity range | 1 pmol to 4 nmol |
| Column material | Hypersil ODS, 5 µm |
| Flow rate (initial) | 0.45 ml/min |
Both primary and secondary amino acids were analyzed in one run.
The method uses UV-DAD at 338 nm and 266 nm, as well as fluorescence detection with specific excitation and emission wavelengths.