The document introduces the HPLC method for analysis of aflatoxins in pistachio nuts.
The document details a High-Performance Liquid Chromatography (HPLC) method for analyzing specific mycotoxins, including aflatoxins G1, G2, B1, B2, M1, and M2, ochratoxin A, zearalenone, and patulin. The analysis is performed on pistachio nuts using an Agilent 1100 Series system equipped with a diode array detector (DAD) and a fluorescence detector (FLD).
The method utilizes reversed-phase chromatography with specific columns such as Hypersil ODS or Lichrospher 100 RP18. Detection limits range from 1 to 5 pg/kg for aflatoxins, with repeatability of retention time over 10 runs being less than 0.12%. The document provides chromatographic conditions, including mobile phase compositions and flow rates, as well as a representative chromatogram.
| Limit of detection (Aflatoxins) | 1-5 pg/kg |
|---|---|
| Repeatability of RT over 10 runs | <0.12 % |
| Linearity of areas (UV-visible DAD) | 1-500 ng |
| Column class for Aflatoxins | Hypersil ODS, 100 ~ 2.1 mm id |
| Flow rate (Aflatoxins) | 0.3 ml/min at 25 °C |
The method analyzes aflatoxins G1, G2, B1, B2, M1, and M2, ochratoxin A, zearalenone, and patulin.
The method analyzes aflatoxins G1, G2, B1, B2, M1, and M2; ochratoxin A; zearalenone; and patuline.
The limit of detection ranges from 1 to 5 pg/kg.