Agilent 1100 Series Analysis of Antioxidants in Chewing Gum Using HPLC

This document introduces the method of analyzing antioxidants in chewing gum.

Agilent 1100 Series Analysis of Antioxidants in Chewing Gum Using HPLC - cover page
Brand
Agilent
Category
Other
Document type
Application Note
Language
English
Pages
2
File format
PDF
File size
160 KB
Published
12 June, 2012
Updated
30 September, 2023
Model
1100 Series
MD5 checksum
96546659883F18D78785247FD7FBCD4C
PDF
Agilent 1100 Series Analysis of Antioxidants in Chewing Gum Using HPLC PDF

About this manual

This document details a High-Performance Liquid Chromatography (HPLC) method for analyzing antioxidants in chewing gum. It covers both natural antioxidants, such as vitamin C and vitamin E, and synthetic antioxidants like BHT, BHA, and propyl gallate. The method utilizes ultrasonic liquid extraction with acetonitrile for sample preparation.

What's inside

  • Abstract
  • Sample preparation
  • Chromatographic conditions
  • HPLC method performance
  • Equipment
  • References

Specifications

Limit of detection0.1-2 ng (injected amount), S/N = 2
Repeatability of RT<0.2 % over 10 runs
Repeatability of areas<1 % over 10 runs

Frequently asked questions

What software is required?

The analysis requires Agilent ChemStation software.

What antioxidants are analyzed in chewing gum using this method?

The method analyzes natural antioxidants like vitamin C and vitamin E, and synthetic antioxidants including BHT, BHA, TBHO, THBP, PG, Ionox-100, NDGA, and TDPA.

What is the flow rate for the HPLC analysis?

The flow rate is set to 0.5 ml/min.

What is the column compartment temperature?

The column compartment is maintained at 30 °C.

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