This brief demonstrates the analysis of various sugars in candy and juice using PAD with post- column pH–changing. The sugars are separated by both ligand exchange and size exclusion mode.
The document describes a method for analyzing sucrose, glucose, galactose, mannose, fructose, mannitol, and sorbitol. The technique utilizes High-Performance Liquid Chromatography (HPLC) combined with a pulsed amperometric detector (PAD) and post-column pH-changing to achieve simple, easy, and highly sensitive detection of sugars in samples like candy and juice.
The setup includes an Agilent 1100 Series system equipped with isocratic pumps, a vacuum degasser, an autosampler, a thermostatted column compartment, and the programmable electrochemical detector (Agilent 1049). The method employs ligand exchange and size exclusion modes for separation.
| Working electrode material | gold |
|---|---|
| Applied Potential Pot1 | 0.65 V |
| Applied Potential Pot2 | -0.95 V |
| Applied Potential Pot3 | 0.15 V |
| Limit of detection | 0.23~0.95 mg/L (S/N = 3) |
| RSD of peak area | 1.0~4.0 % |
The method analyzes sucrose, glucose, galactose, mannose, fructose, mannitol, and sorbitol.
The analysis was demonstrated using candy and apple juice samples.