This Application Note describes the isolation of three antianginal drugs (nifedipine, nisoldipine, and nimodipine) from a mixture as an example application for purification of pharmaceutical drugs using the Agilent 1100 Series purification1,2 system PS (preparative scale). The goal of the application was to purify 20 mg of each compound in a single run and to get three fractions containing only the desired substances.
This document describes an application for the purification of pharmaceutical drugs, specifically nifedipin, nimodipin, and nisoldipin. The authors demonstrate the isolation of these compounds from a mixture using the Agilent 1100 Series purification system equipped with a mass-selective detector (MSD) for mass-based fraction collection. The goal was to purify 20 mg of each compound in a single run.
| Flow rate | 25 mL/min |
|---|---|
| Column dimensions | 21.2 x 50 mm |
| Injection volume | 500 pL |
| Nifedipin molecular mass | 346.34 |
| Nimodipin molecular mass | 418.45 |
| Nisoldipin molecular mass | 388.42 |
| Nimodipin trigger mass | 342 |
The goal was to purify 20 mg of each compound in a single run and obtain three fractions containing only the desired substances.
The system was controlled using Agilent ChemStation (rev. A.09.01) and Purification/HighThruput software (rev. A.01.01).