This Application Note describes a more sophisticated approach to peak-based fraction collection by using the parameters up and down slope, upper threshold and maximum peak duration.
This document explains how to perform sophisticated peak-based fraction collection using the Agilent 1100 Series purification system. It details the use of parameters such as up and down slope, upper threshold, and maximum peak duration to improve triggering decisions compared to standard threshold-only methods.
The text covers the theoretical basis of slope triggering, the function of the fraction preview tool in the Agilent ChemStation for optimizing settings, and provides practical applications for separating steep and shallow peaks. It also offers tips on setting reliable values for different chromatographic conditions.
Reliable fraction collection cannot be expected with slope settings less than approximately 10 mAU/s.
Triggering decisions based on up and down slope are ignored until the signal falls back below the upper threshold.